Antibiotic resistance and virulence of enterococci isolates from healthy humans in Tunisia

Autor: Ben Sallem, R; Klibi, N; Klibi, A; Ben Said, L; Dziri, R; Boudabous, A; Torres Manrique, Carmen; Ben Slama, K; 

Tipo de documento: Artículo de revista

Revista: Annals of Microbiology. ISSN: 1590-4261. Año: 2016. Número: 2. Volumen: 66. Páginas: 717-725.

JCR (datos correspondientes al año 2014):
Science  Área: BIOTECHNOLOGY & APPLIED MICROBIOLOGY  Quartil: Q4  Lugar área: 129/162  F. impacto: 0,990 
Science  Área: MICROBIOLOGY  Quartil: Q4  Lugar área: 129/162  F. impacto: 0,990 

SCIMAGO (datos correspondientes al año 2014):
,388  SNIP: ,72 



  • Aarestrup, F.M., Agerso, Y., Gerner-Smidt, P., Madsen, M., Jensen, L.B., Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark. Diagn (2000) Microbiol Infect Dis, 37, pp. 127-137. , COI: 1:CAS:528:DC%2BD3cXktV2qsLo%3D
  • Acar, J., Rostel, B., Antimicrobial resistance: an overview (2001) Rev Sci Tech, 20, pp. 797-810. , COI: 1:STN:280:DC%2BD3Mnos1OhsA%3D%3D, PID: 11732423
  • Arias, C.A., Robredo, B., Singh, K.V., Torres, C., Panesso, D., Murray, B.E., Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. durans (2006) J Clin Microbiol, 44, pp. 1567-1570. , COI: 1:CAS:528:DC%2BD28XkvVamur4%3D, PID: 16597896
  • Arias, C.A., Contreras, G.A., Murray, B.E., Management of multidrug-resistant enterococcal infections (2010) Clin Microbiol Infect, 16, pp. 555-562. , COI: 1:CAS:528:DC%2BC3cXot1GmsrY%3D, PID: 20569266
  • Barreto, A., Guimarães, B., Radhouani, H., Araújo, C., Gonçalves, A., Gaspar, E., Rodrigues, J., Poeta, P., Detection of antibiotic resistant E. coli and Enterococcus spp. in stool of healthy growing children in Portugal (2009) J Basic Microbiol, 49, pp. 503-519. , COI: 1:CAS:528:DC%2BC3cXht1Ohu78%3D, PID: 19810048
  • Bik, E.M., Composition and function of the human-associated microbiota (2009) Nutr Rev, 67, pp. S164-S171. , PID: 19906220
  • Birri, D.J., Brede, D.A., Tessema, G.T., Nes, I.F., Bacteriocin production, antibiotic susceptibility and prevalence of haemolytic and gelatinase activity in faecal lactic acid bacteria isolated from healthy Ethiopian infants (2013) Microb Ecol, 65, pp. 504-516. , COI: 1:CAS:528:DC%2BC3sXitFekur8%3D, PID: 23184155
  •, Boehm AB, Sassoubre LM (2014) Enterococci as indicators of environmental fecal contamination. In Gilmore MS, Clewell DB, Ike Y, Shankar N (eds). Enterococci: from commensals to leading causes of drug resistant infection [Internet]. Massachusetts Eye and Ear Infirmary, Boston, Available from:Comité de l’Antibiogramme de la Société Française de Microbiologie (2015) Edition Janvier, p. 2015
  • Cetinkaya, Y., Falk, P., Mayhall, C., Vancomycin-resistant enterococci (2000) Clin Microbiol Rev, 13, pp. 686-707. , COI: 1:CAS:528:DC%2BD3cXnslCgtbg%3D, PID: 11023964
  • Chapman, T.A., Wu, X.Y., Barchia, I., Bettelheim, K.A., Driesen, S., Trott, D., Wilson, M., Chin, J.J., Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine (2006) Appl Environ Microbiol, 72, pp. 4782-4795. , COI: 1:CAS:528:DC%2BD28XnsVaqsb8%3D, PID: 16820472
  • Clementi, F., Aquilanti, L., Recent investigations and updated criteria for the assessment of antibiotic resistance in food lactic acid bacteria (2011) Anaerobe, 17, pp. 394-398. , COI: 1:CAS:528:DC%2BC38XhtlSntw%3D%3D, PID: 21515393
  • Performance Standards for antimicrobial susceptibility testing
  • twenty-second informational Supplement (2015) CLSI document, pp. M100-M122. , Clinical and Laboratory Standards Institute, Wayne, PA
  • Creti, R., Imperi, M., Bertuccini, L., Fabretti, F., Orefici, G., Di Rosa, R., Baldassarri, L., Survey of virulence determinants among Enterococcus faecalis isolated from different sources (2004) J Med Microbiol, 53, pp. 13-20. , COI: 1:CAS:528:DC%2BD2cXjtFCjsbY%3D, PID: 14663100
  • Del Campo, R., Ruiz-Garbajosa, P., Sanchez-Moreno, M.P., Baquero, F., Torres, C., Canton, R., Coque, T.M., Antimicrobial resistance in recent fecal enterococci from healthy volunteers and food handlers in Spain: genes and phenotypes (2003) Microb Drug Resist, 9, pp. 47-60. , PID: 12705683
  • Donabedian, S.M., Thal, L.A., Hershberger, E., Perri, M.B., Chow, J.W., Bartlett, P., Jones, R., Zervos, M.J., Molecular characterization of gentamicin-resistant enterococci in the United States: evidence of spread from animals to humans through food (2003) J Clin Microbiol, 41, pp. 1109-1113. , COI: 1:CAS:528:DC%2BD3sXisF2ms7s%3D, PID: 12624037
  • Dutka-Malen, S., Evers, S., Courvalin, P., Detection of glycopeptides resistance genotypes and identification to the species level of clinically relevant enterococci by PCR (1995) J Clin Microbiol, 33, pp. 24-27. , COI: 1:CAS:528:DyaK2MXjsVGnsrw%3D, PID: 7699051
  • Fisher, K., Phillips, C., The ecology, epidemiology and virulence of Enterococcus (2009) Microbiology, 155, pp. 1749-1757. , COI: 1:CAS:528:DC%2BD1MXotVaqsL8%3D, PID: 19383684
  • Franz, C.M., Muscholl-Silberhorn, A.B., Yousif, N.M., Vancanneyt, M., Swings, J., Holzapfel, W.H., Incidence of virulence factors and antibiotic resistance among enterococci isolated from food (2001) Appl Environ Microbiol, 67, pp. 4385-4389. , COI: 1:CAS:528:DC%2BD3MXmslWit74%3D, PID: 11526054
  • Freitas, A.R., Coque, T.M., Novais, C., Hammerum, A.M., Lester, C.H., Zervos, M.J., Donabedian, S., Peixe, L., Human and swine hosts sharevancomycin-resistant Enterococcus faecium CC17 and CC5 and Enterococcus faecalis CC2 clonal clusters harboring Tn1546 on indistinguishable plasmids (2011) J Clin Microbiol, 49 (3), pp. 925-931. , COI: 1:CAS:528:DC%2BC3MXlt1WjurY%3D, PID: 21227995
  • Hammerum, A.M., Enterococci of animal origin and their significance for public health (2012) Clin Microbiol Infect, 18, pp. 619-625. , COI: 1:CAS:528:DC%2BC38XhtFOhtrzP, PID: 22487203
  • Heuer, O.E., Hammerum, A.M., Collignon, P., Wegener, H.C., Human health hazard from antimicrobial-resistant enterococci in animals and food (2006) Clin Infect Dis, 43, pp. 911-916. , PID: 16941376
  • Jackson, C.R., Fedorka-Cray, P.J., Barrett, J.B., Use of a genus and species-specific multiplex PCR for identification of enterococci (2004) J Clin Microbiol, 42, pp. 3558-3565. , COI: 1:CAS:528:DC%2BD2cXnt1Ogt70%3D, PID: 15297497
  • Kirtzalidou, E.I., Mitsou, E.K., Pramateftaki, P., Kyriacou, A., Screening faecal enterococci from Greek healthy infants for susceptibility to antimicrobial agents (2012) Microb Drug Resist, 18, pp. 578-585. , COI: 1:CAS:528:DC%2BC38Xhs12itLvF, PID: 22827719
  • Klibi, N., Gharbi, S., Masmoudi, A., Ben Slama, K., Poeta, P., Zarazaga, M., Fendri, C., Torres, C., Antibiotic resistance and mechanisms implicated in clinical Enterococci in a Tunisian hospital (2006) J Chemother, 18 (1), pp. 20-26. , COI: 1:CAS:528:DC%2BD28XjtlGnu70%3D, PID: 16572889
  • Klibi, N., Ben Slama, K., Sáenz, Y., Masmoudi, A., Zanetti, S., Sechi, L.A., Boudabous, A., Torres, C., Detection of virulence factors in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolates from a Tunisian hospital (2007) Can J Microbiol, 53, pp. 372-379. , COI: 1:CAS:528:DC%2BD2sXntV2msrw%3D, PID: 17538646
  • Klibi, N., Sáenz, Y., Zarazaga, M., Ben Slama, K., Masmoudi, A., Ruiz-Larrea, F., Boudabous, A., Torres, C., Polymorphism in pbp5 gene detected in clinical Enterococcus faecium strains with different ampicillin MICs from a Tunisian hospital (2008) J Chemother, 20, pp. 436-440
  • Klibi, N., Ben Slimen, N., Fhoula, I., López, M., Ben Slama, K., Daffonchio, D., Boudabous, A., Ouzari, H., Genotypic diversity, antibiotic resistance and bacteriocin production of enterococci isolated from rhizospheres (2012) Microb Environ, 27, pp. 533-537
  • Klibi, N., Ben Said, L., Jouini, A., Ben Slama, K., López, M., Ben Sallem, R., Boudabous, A., Torres, C., Species distribution, antibiotic resistance and virulence traits in enterococci from meat in Tunisia (2013) Meat Sci, 93, pp. 675-680. , COI: 1:CAS:528:DC%2BC3sXhvVSrtw%3D%3D, PID: 23273480
  • Klibi, N., Aouini, R., Borgo, F., Ben Said, L., Ferrario, C., Dziri, R., Boudabous, A., Ben Slama, K., Antibiotic resistance and virulence of faecal enterococci isolated from food-producing animals in Tunisia (2015) Ann Microbiol, 65, pp. 695-702
  • Laverde Gomez, J.A., Van Schaik, W., Freitas, A.R., Coque, T.M., Weaver, K.E., Francia, M.V., Witte, W., Werner, G., Multiresistance megaplasmid pLG1 bearing a hyl Efm genomic island in hospital Enterococcus faecium isolates (2010) Int J Med Microbiol, 301, pp. 165-175. , PID: 20951641
  • Lebreton, F., Van Schaik, W., McGuire, A.M., Godfrey, P., Griggs, A., Mazumdar, V., Corander, J., Gilmore, M.S., Emergence of epidemic multidrug-resistant Enterococcus faecium from animal and commensal strains (2013) mBio, 4, pp. 1-10
  • Lester, C.H., Frimodt-Moller, N., Sorensen, T.L., Monnet, D.L., Hammerum, A.M., In vivo transfer of the vanA resistance gene from an Enterococcus faecium isolate of animal origin to an E. faecium isolate of human origin in the intestines of human volunteers (2006) Antimicrob Agents Chemother, 50, pp. 596-599. , COI: 1:CAS:528:DC%2BD28XhsFegtLY%3D, PID: 16436715
  • López, M., Tenorio, C., Torres, C., Study of vancomycin resistance in faecal enterococci from healthy humans and dogs in Spain a decade after the avoparcin ban in Europe (2013) Zoonoses Public Health, 60, pp. 160-167. , PID: 22646722
  • Magiorakos, A.P., Srinivasan, A., Carey, R.B., Carmeli, Y., Falagas, M.E., Giske, C.G., Harbarth, S., Monnet, D.L., Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance (2012) Clin Microbiol Infect, 18, pp. 268-281. , COI: 1:CAS:528:DC%2BC38XmvValtb4%3D, PID: 21793988
  • Mazzulli, T., King, S.M., Richardson, S.E., Bacteremia due to beta-lactamase producing Enterococcus faecalis with high-level resistance to gentamicin in a child with Wiskott-Aldrich syndrome (1992) Clin Infect Dis, 14, pp. 780-781. , COI: 1:STN:280:DyaK383itlamsw%3D%3D, PID: 1562666
  • Mitsou, E.K., Kirtzalidou, E., Pramateftaki, P., Kyriacou, A., Antibiotic resistance in faecal microbiota of Greek Healthy infants (2010) Benefic Microbes, 1, pp. 297-306. , COI: 1:CAS:528:DC%2BC3MXhsFGru7nM
  • Nilsson, O., Vancomycin resistant enterococci in farm animals—occurrence and importance (2012) Infect Ecol Epidemiol, 2, pp. 1-8
  • Novais, C., Coque, T.M., Sousa, J.C., Peixe, L.V., Antimicrobial resistance among faecal enterococci from healthy individuals in Portugal (2006) Clin Microbiol Infect, 12, pp. 1131-1134. , COI: 1:CAS:528:DC%2BD28XhtF2lsb7K, PID: 17002614
  • Poeta, P., Costa, D., Rodrigues, J., Torres, C., Study of faecal colonization by vanA-containing Enterococcus strains in healthy humans, pets, poultry and wild animals in Portugal (2005) J Antimicrob Chemother, 55 (2), pp. 278-280. , COI: 1:CAS:528:DC%2BD2MXovV2jtQ%3D%3D, PID: 15649991
  • Poeta, P., Costa, D., Rodrigues, J., Torres, C., Antimicrobial resistance and the mechanisms implicated in faecal enterococcal strains from healthy humans, poultry and pets in Portugal (2006) Int J Antimicrob Agents, 27, pp. 131-137. , COI: 1:CAS:528:DC%2BD28XotVeqtg%3D%3D, PID: 16388931
  • Portillo, A., Ruiz-Larrea, F., Zarazaga, M., Alonso, A., Martinez, J.L., Torres, C., Macrolide resistance genes in Enterococcus spp (2000) Antimicrob Agents Chemother, 44 (4), pp. 967-971. , COI: 1:CAS:528:DC%2BD3cXitFOlurc%3D, PID: 10722498
  • Silva, N., Igrejas, G., Gonçalves, A., Poeta, P., Commensal gut bacteria: distribution of Enterococcus species and prevalence of E. coli phylogenetic groups in animals and humans in Portugal (2012) Ann Microbiol, 62, pp. 449-459
  • Turabelidze, D., Kotetishvili, M., KregerA, M.J., Sulakvelidze, A., Improved pulsed-field gel electrophoresis for typingvancomycin-resistant enterococci (2000) J Clin Microbiol, 38, pp. 4242-4245. , COI: 1:CAS:528:DC%2BD3MXhs1Slu7k%3D, PID: 11060099
  • Upadhyaya, G.P., Lingadevaru, U.B., Ligegowda, R.K., Comparative study among clinical and commensal isolates of Enterococcus faecalis for presence ofesp gene and biofilm production (2011) J Infect Dev Ctries, 28, pp. 365-369
  • Van Tyne, D., Martin, M.J., Gilmore, M.S., Structure, function, and biology of the Enterococcus faecalis cytolysin (2013) Toxins, 5 (5), pp. 895-911. , PID: 23628786
  • Wegener, H.C., Aarestrup, F.M., Jensen, L.B., Hammerum, A.M., Bager, F., Use of antimicrobial growth promoters in food animals and Enterococcus faecium resistance to therapeutic antimicrobial drugs in Europe (1999) Emerg Infect Dis, 5, pp. 329-335. , COI: 1:STN:280:DyaK1M3ns1Krtw%3D%3D, PID: 10341169