Genotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clones with high-level mupirocin resistance

Autor: González-Domínguez, M.; Seral, C.; Potel, C.; Sáenz, Y.; Álvarez, M.; Torres Manrique, Carmen; Castillo, F.J.; 

Tipo de documento: Artículo de revista

Revista: Diagnostic Microbiology and Infectious Disease. ISSN: 0732-8893. Año: 2016. Número: 2. Volumen: 85. Páginas: 213-217.

JCR (datos correspondientes al año 2014):
Science  Área: INFECTIOUS DISEASES  Quartil: Q3  Lugar área: 44/78  F. impacto: 2,457 
Science  Área: MICROBIOLOGY  Quartil: Q3  Lugar área: 44/78  F. impacto: 2,457 

SCIMAGO (datos correspondientes al año 2014):
1,021  SNIP: ,918 



  • Aarestrup, F.M., Agerso, Y., Gerner-Smidt, P., Madsen, M., Jensen, L.B., Comparison of antimicrobial resistance phenotypes and resistance genes in Enterococcus faecalis and Enterococcus faecium from humans in the community, broilers, and pigs in Denmark (2000) Diagn Microbiol Infect Dis, 37, pp. 127-137
  • Aktas, Z., Aridogan, A., Kayacan, C.B., Aydin, D., Resistance to macrolide, lincosamide and streptogramin antibiotics in staphylococci isolated in Istanbul, Turkey (2007) J Microbiol, 45, pp. 286-290
  • Argudín, M.A., Mendoza, M.C., Méndez, F.J., Martín, M.C., Guerra, B., Rodicio, M.R., Clonal complexes and diversity of exotoxin gene profiles in methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from patients in a Spanish hospital (2009) J Clin Microbiol, 47, pp. 2097-2105
  • Choi, S.M., Kim, S.H., Kim, H.J., Lee, D.G., Choi, J.H., Yoo, J.H., Multiplex PCR for the detection of genes encoding aminoglycoside modifying enzymes and methicillin resistance among Staphylococcus species (2003) J Korean Med Sci, 18, pp. 631-636
  • Performance standards for antimicrobial susceptibility testing (2014) CLSI document M100-S24, , Twenty-Fourth Informational Supplement
  • Cuevas, O., Cercenado, E., Goyanes, M.J., Vindel, A., Trincado, P., Boquete, T., Staphylococcus spp. in Spain: present situation and evolution of antimicrobial resistance (1986-2006) (2008) Enferm Infecc Microbiol Clin, 26, pp. 269-277
  • Daskalaki, M., Otero, J.R., Chaves, F., Molecular characterization of resistance to mupirocin in methicillin-resistant Staphylococcus aureus isolates in a tertiary hospital in Spain (2009) J Antimicrob Chemother, 63, pp. 826-828
  • González-Domínguez, M., Seral, C., Sáenz, Y., Salvo, S., Gude, M.J., Porres-Osante, N., Epidemiological features, resistance genes, and clones among community-onset methicillin-resistant Staphylococcus aureus (CO-MRSA) isolates detected in northern Spain (2012) Int J Med Microbiol, 302, pp. 320-326
  • González-Domínguez, M., Seral, C., Potel, C., Constenla, L., Algarate, S., Gude, M.J., Antimicrobial resistance, virulence factors and genetic lineages of hospital-onset methicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza (2015) Enferm Infecc Microbiol Clin, 33, pp. 590-596
  • Jarraud, S., Mougel, C., Thioulouse, J., Lina, G., Meugnier, H., Forey, F., Relationships between Staphylococcus aureus genetic background, virulence factors, agr groups (alleles), and human disease (2002) Infect Immun, 70, pp. 631-641
  • Larsen, A.R., Stegger, M., Sorum, M., Spa typing directly from a mecA, spa and pvl multiplex PCR assay-a cost-effective improvement for methicillin-resistant Staphylococcus aureus surveillance (2008) Clin Microbiol Infect, 14, pp. 611-614
  • Lee, A.S., Macedo-Vinas, M., Francois, P., Renzi, G., Vernaz, N., Schrenzel, J., Trends in mupirocin resistance in meticillin-resistant Staphylococcus aureus and mupirocin consumption at a tertiary care hospital (2011) J Hosp Infect, 77, pp. 360-362
  • Leski, T.A., Gniadkowski, M., Skoczynska, A., Stefaniuk, E., Trzcinski, K., Hryniewicz, W., Outbreak of mupirocin-resistant staphylococci in a hospital in Warsaw, Poland, due to plasmid transmission and clonal spread of several strains (1999) J Clin Microbiol, 37, pp. 2781-2788
  • Lina, G., Quaglia, A., Reverdy, M.E., Leclercq, R., Vandenesch, F., Etienne, J., Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among staphylococci (1999) Antimicrob Agents Chemother, 43, pp. 1062-1066
  • Lozano, C., Porres-Osante, N., Crettaz, J., Rojo-Bezares, B., Benito, D., Olarte, I., Changes in genetic lineages, resistance, and virulence in clinical methicillin-resistant Staphylococcus aureus in a Spanish hospital (2013) J Infect Chemother, 19, pp. 233-242
  • McDougal, L.K., Fosheim, G.E., Nicholson, A., Bulens, S.N., Limbago, B.M., Shearer, J.E., Emergence of resistance among USA300 methicillin-resistant Staphylococcus aureus isolates causing invasive disease in the United States (2010) Antimicrob Agents Chemother, 54, pp. 3804-3811
  • Menegotto, F., González-Cabrero, S., Lorenzo, B., Cubero, A., Cuervo, W., Gutiérrez, M.P., Molecular epidemiology of methicillin-resistant Staphylococcus aureus in a Spanish hospital over a 4-year period: clonal replacement, decreased antimicrobial resistance, and identification of community-acquired and livestock-associated clones (2012) Diagn Microbiol Infect Dis, 74, pp. 332-337
  • Pérez-Roth, E., López-Aguilar, C., Alcoba-Flórez, J., Méndez-Álvarez, S., High-level mupirocin resistance within methicillin-resistant Staphylococcus aureus pandemic lineages (2006) Antimicrob Agents Chemother, 50, pp. 3207-3211
  • Pérez-Roth, E., Kwong, S.M., Alcoba-Florez, J., Firth, N., Méndez-Álvarez, S., Complete nucleotide sequence and comparative analysis of pPR9, a 41.7-kilobase conjugative staphylococcal multiresistance plasmid conferring high-level mupirocin resistance (2010) Antimicrob Agents Chemother, 54, pp. 2252-2257
  • Pérez-Roth, E., Armas-González, E., Alcoba-Florez, J., Méndez-Álvarez, S., PCR-based amplification of heterogeneous IS257-ileS2 junctions for molecular monitoring of high-level mupirocin resistance in staphylococci (2011) J Antimicrob Chemother, 66, pp. 471-475
  • Pérez-Roth, E., Potel-Alvarellos, C., Espartero, X., Constela-Caramés, L., Méndez-Álvarez, S., Álvarez-Fernández, M., Molecular epidemiology of plasmid-mediated high-level mupirocin resistance in methicillin-resistant Staphylococcus aureus in four Spanish health care settings (2013) Int J Med Microbiol, 303, pp. 201-204
  • Rodríguez-Baño, J., Bischofberger, C., Álvarez-Lerma, F., Asensio, A., Delgado, T., García-Arcal, D., Surveillance and control of methicillin-resistant Staphylococcus aureus in Spanish hospitals. A GEIH-SEIMC and SEMPSPH consensus document (2008) Enferm Infecc Microbiol Clin, 26, pp. 285-298
  • Schmitz, F.J., Jones, M.E., Antibiotics for treatment of infections caused by MRSA and elimination of MRSA carriage. What are the choices? (1997) Int J Antimicrob Agents, 9, pp. 1-19
  • Simor, A.E., Stuart, T.L., Louie, L., Watt, C., Ofner-Agostini, M., Gravel, D., Mupirocin-resistant, methicillin-resistant Staphylococcus aureus strains in Canadian hospitals (2007) Antimicrob Agents Chemother, 51, pp. 3880-3886
  • Sutherland, R., Boon, R.J., Griffin, K.E., Masters, P.J., Slocombe, B., White, A.R., Antibacterial activity of mupirocin (pseudomonic acid), a new antibiotic for topical use (1985) Antimicrob Agents Chemother, 27, pp. 495-498
  • Udo, E.E., Jacob, L.E., Mathew, B., Genetic analysis of methicillin-resistant Staphylococcus aureus expressing high- and low-level mupirocin resistance (2001) J Med Microbiol, 50, pp. 909-915
  • Woodford, N., Watson, A.P., Patel, S., Jevon, M., Waghorn, D.J., Cookson, B.D., Heterogeneous location of the mupA high-level mupirocin resistance gene in Staphylococcus aureus (1998) J Med Microbiol, 47, pp. 829-835
  • Yanagisawa, T., Lee, J.T., Wu, H.C., Kawakami, M., Relationship of protein structure of isoleucyl-tRNA synthetase with pseudomonic acid resistance of Escherichia coli. A proposed mode of action of pseudomonic acid as an inhibitor of isoleucyl-tRNA synthetase (1994) J Biol Chem, 269, pp. 24304-24309