Virulence characteristics and genetic background of ESBL-producing Klebsiella pneumoniae isolates from wastewater

Autor: Atmani, S.M.; Messai, Y.; Alouache, S.; Fernandez, R.; Estepa Pérez, VanesaTorres Manrique, Carmen; Bakour, R.; 

Tipo de documento: Artículo de revista

Revista: Fresenius Environmental Bulletin. ISSN: 1018-4619. Año: 2015. Número: 1. Volumen: 24. Páginas: 103-112.

JCR (datos correspondientes al año 2014):
Science  Área: ENVIRONMENTAL SCIENCES  Quartil: Q4  Lugar área: 217/221  F. impacto: 0,378 

SCIMAGO (datos correspondientes al año 2014):
,212  SNIP: ,299 



  • Podschun, R., Ullmann, U., Klebsiella spp. As nosocomial pathogens: Epidemiology, taxonomy, typing methods and pathogenicity factors (1998) Clin. Microbiol. Rev., 11, pp. 589-603
  • Yu, V.L., Hansen, D.S., Ko, W.C., Sagnimeni, A., Klugman, K.P., Von Gottberg, A., Goossens, H., Benedi, V.J., Virulence characteristics of Klebsiella and clinical manifestations of K. Pneumoniae bloodstream infections (2007) Emerg. Infect. Dis., 13, pp. 986-993
  • Bachman, M.A., Oyler, J.E., Burns, S.H., Caza, M., Lppine, F., Dozois, C.M., Weiser, J.N., Klebsiellapneumoniae yersiniabactin promotes respiratory tract Infection through evasion of lipocalin 2 (2011) Infect. Immun., 79, pp. 3309-3316
  • Sanchez, C.J., Mende, K., Beckius, M.L., Akers, K.S., Romano, D.R., Wenke, J.C., Murray, C.K., Biofilm formation by clinical isolates and the implications in chronic infections (2013) BMC Infect. Dis., 13, p. 47
  • Kumar, V., Sun, P., Vamathevan, J.Y., Ingraham, K., Palmer, L., Huang, J., Brown, J.R., Comparative genomics of Klebsiellapneumoniae strains with different antibiotic resistance profiles (2011) Antimicrob. Agents Chemother., 55, pp. 4267-4276
  • Ramos, P.I.P., Picão, R.C., De Almeida, L.G.P., Lima, N.C.B., Girardello, R., Vivan, A.C.P., Xavier, D.E., Nicolás, M.F., Comparative analysis of the complete genome of KPC-2-producing Klebsiellapneumoniae Kpl3 reveals remarkable genome plasticity and a wide repertoire of virulence and resistance mechanisms (2014) BMC Genomics., 15, p. 54
  • Emmanuel, E., Perrodin, Y., Keck, G., Blanchard, J.M., Vermande, P., Ecotoxicological risk assessment of hospital wastewater: A proposed framework for raw effluents discharging into urban sewernetwork (2005) J. Hazard. Mater., 117, pp. 1-11
  • Duong, H.A., Pham, N.H., Nguyen, H.T., Hoang, T.T., Pham, H.V., Pham, V.C., Berg, M., Alder, A.C., Occurrence, fate and antibiotic resistance of fluoroquinoloneantibacterials in hospital wastewaters in Hanoi, Vietnam (2008) Chemosphere, 72, pp. 968-973
  • Wibberg, D., Szczepanowski, R., Eikmeyer, F., Pühler, A., Schlüter, A., The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. Coli isolates (2013) Plasmid., 69, pp. 127-137
  • Szczepanowski, R., Braun, S., Riedel, V., Schneiker, S., Krahn, I., Pühler, A., Schlüter, A., The 120 592 bpIncF plasmid pRSB107 isolated from a sewage-treatment plant encodes nine different antibiotic-resistance determinants, two iron-acquisition systems and other putative virulence-associated functions (2005) Microbiology, 151, pp. 1095-1111
  • (2010) CA-SFM (Antibiogram Committee of French Society for Microbiology), ,
  • Messai, Y., Iabadene, H., Benhassine, T., Alouache, S., Tazir, M., Gautier, V., Arlet, G., Bakour, R., Prevalence and characterization of extended-spectrum p-lactamases in Klebsiellapneumoniae in Algiers hospitals (Algeria) (2008) Pathol. Biol., 56, pp. 319-325
  • Gautom, R.K., Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli 0157:H7 and other Gram-negative organisms in 1 Day (1997) J. Clin. Microbiol., 35, pp. 2977-2980
  • Fernández-Pérez, R., Torres, C., Sanz, S., Ruiz-Larrea, F., Strain typing of acetic acid bacteria responsible for vinegar production by the submerged elaboration method (2010) Food-Microbiol., 27, pp. 973-978
  • Brisse, S., Van Himbergen, T., Kusters, K., Verhoef, J., Development of a rapid identification method for Klebsiellapneumoniae phylogenetic groups and analysis of 420 clinical isolates (2004) Clin. Microbiol. Infect., 10, pp. 942-945
  • Diancourt, L., Passet, V., Verhoef, J., Grimont, P.A.D., Brisse, S., Multilocus sequence typing of Klebsiellapneumoniaenosocomial isolates (2005) J. Clin. Microbiol., 48, pp. 4178-4182
  • Mishra, M., Thakar, Y.S., Pathak, A.A., Haemagglutination, haemolysinproduction and serum resistance of Proteus and related species isolated from clinical sources (2001) Indian J. Med. Microbiol., 19, pp. 5-11
  • Fierer, J., Finley, E., Braude, A.I., A plaque assay on agar for detection of Gram-negative bacilli sensitive to complement (1972) J. Immunol., 109, pp. 1156-1158
  • Maldonado, N.C., Silva De Ruiz, C., Cecilia, M., Nader-Macias, M.E., A simple technique to detect Klebsiella biofilm-forming-strains. Inhibitory potential of Lactobacillusfermentum CRL1058 whole cells and products (2007) Communicating Current Research and Educational Topics and Trends in Applied Microbiology, pp. 52-59. , A Méndez-Vilas eds
  • El Fertas-Aissani, R., Messai, Y., Alouache, S., Bakour, R., Virulence profiles and antibiotic susceptibility patterns of Klebsiellapneumoniae strains isolated from different clinical specimens (2013) Pathol. Biol., 61, pp. 209-216
  • Kado, C.I., Liu, S.T., Rapid procedure for detection and isolation of large and small plasmids (1981) J. Bacterid., 45, pp. 1365-1373
  • Carattoli, A., Bertini, A., Villa, L., Falbo, V., Hopkins, K.L., Threlfall, E.J., Identification of plasmids by PCR-based replicon typing (2005) J. Microbiol. Methods., 63, pp. 219-228
  • Reed, L.J., Muench, H., A simple method of estimating fifty percent endpoints (1938) Am. J. Hyg., 27, pp. 493-497
  • Podschun, R., Pietsch, S., Höller, C., Ullmann, U., Incidence of Klebsiella.species in surface waters and their expression of virulence factors (2001) Appl. Env. Microbiol., 67, pp. 3325-3327
  • Tarkkanen, A.M., Virkola, R., Clegg, S., Korhonen, T.K., Binding of the type 3 fimbriae of Klebsiellapneumoniae to human endothelial and urinary bladder cells (1997) Infect. Immun., 65, pp. 1546-1549
  • Schroll, C., Barken, K.B., Krogfelt, K.F., Starve, C., Role of type 1 and type 3 fimbriae in Klebsiellapneumoniae biofilm formation (2010) BMC Microbiol., 179, pp. 1-10
  • Brisse, S., Fevre, C., Passet, V., Issenhuth-Jeanjean, S., Tournebize, R., Diancourt, L., Grimont, P., Virulent clones of Klebsiellapneumoniae: Identification and evolutionary scenario based on genomic and phenotypic characterization (2009) PLOS ONE, 4, p. e4982
  • Koczura, R., Kaznowski, A., Occurrence of the Yersinia high-pathogenicity island and iron uptake systems in clinical isolates of Klebsiellapneumoniae (2003) Microb. Pathog., 35, pp. 197-202
  • Chaturvedi, K.S., Hung, C.S., Crowley, J.R., Stapleton, A.E., Henderson, J.P., Thesiderophoreyersiniabactin binds copper to protect pathogens during infection (2012) Nat. Chem. Biol., 8, pp. 731-736
  • Schubert, S., Cuenca, S., Fisher, D., Heesemann, J., High-pathogenicity island of Yersinia pestis in Enterobacteriacea isolated from blood cultures and urine samples: Prevalence and functional expression (2000) J. Infect. Dis., 182, pp. 1268-1271
  • Bach, S., De Almeida, A., Carniel, E., The Yersinia high-pathogenicity island is present in different members of the family Enterobacteriaceae (2000) FEMS Microbiol. Lett., 183, pp. 289-294
  • Putze, J., Hennequin, C., Nougayréde, J.P., Zhang, W., Homburg, S., Karch, H., Bringer, M.A., Dobrindt, U., Genetic structure and distribution of the colibactin genomic island among members of the family Enterobacteriaceae (2009) Infect. Immun., 77, pp. 4696-4703
  • Turton, J.F., Perry, C., Elgohari, S., Hampton, C.V., PCR characterization and typing of Klebsiellapneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets (2010) J. Med. Microbiol., 59, pp. 541-547
  • Izquierdo, L., Coderch, N., Piqué, N., Bedini, E., Corsaro, M.M., Merino, S., Fresno, S., Regué, M., The KlebsiellapneumoniaewabG gene: Role in biosynthesis of the core lipopolysaccharide and virulence (2003) J. Bacteriol., 185, pp. 7213-7221
  • Regué, M., Hita, B., Piqué, N., Izquierdo, L., Merino, S., Fresno, S., Benedi, V.J., Toməs, J.M., A gene, uge, is essential for Klebsiellapneumoniae virulence (2004) Infect. Immun., 72, pp. 54-61
  • Sahly, H., Aucken, H., Benedi, V.J., Forestier, C., Fussing, V., Hansen, D.S., Ofek, I., Ullmann, U., Increased serum resistance in Klebsiellapneumoniae strains producing extendedspectrum p-lactamases (2004) Antimicrob. Agents Chemother., 48, pp. 3477-3482
  • Maroncle, N., Rich, C., Forestier, C., The role of Klebsiellapneumoniae urease in intestinal colonization and resistance to gastrointestinal stress (2006) Res. Microbiol., 157, pp. 184-193
  • Gundogan, N., Citak, S., Yalci, E., Virulence properties of extended spectrum beta-lactamase-producing Klebsiella species in meat samples (2011) J. Food. Protect., 74, pp. 559-564
  • Villa, L., Garcia-Fernandez, A., Fortini, D., Carattoli, A., Replicon sequence typing of IncF plasmids carrying virulence and resistance determinants (2010) J. Antimicrob. Chemother., 65, pp. 2518-2529
  • Chan, W.W., Peirano, G., Smyth, D.J., Pitout, J.D.D., The characteristics of Klebsiellapneumoniae that produce KPC-2 imported from Greece (2013) Diagn. Microbiol. Infect. Dis., 75, pp. 317-319
  • Tang, H.L., Chiang, M.K., Liou, W.J., Chen, Y.T., Peng, H.L., Chiou, C.S., Liu, K.S., Lai, Y.C., Correlation between Klebsiellapneumoniae carrying pLVPK-derived loci and abscess formation (2010) Eur. J. Clin. Microbiol. Infect. Dis., 29, pp. 689-698
  • Dolejska, M., Villa, L., Dobiasova, H., Fortini, D., Feudi, C., Carattoli, A., Plasmid content of a clinically relevant Klebsiellapneumoniae clone from the Czech republic producing CTX-M-15 and QnrBl (2013) Antimicrob. Agents Chemother., 57, pp. 1073-1076
  • Mshana, S.E., Hain, T., Domann, E., Lyamuya, E.F., Chakraborty, T., Imirzalioglu, C., Predominance of Klebsiellapneumoniae ST14 carrying CTX-M-15 causing neonatal sepsis in Tanzania (2013) BMC Infect. Dis., 13, p. 466
  • Oteo, J., Hernández, J.M., Espasa, M., Fleites, A., Sáez, D., Bautista, V., Pérez-Vázquez, M., Campos, J., Emergence of OXA-48-producing Klebsiellapneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain (2013) J. Antimicrob. Chemother., 68, pp. 317-321
  • Quiñones, D., Valverde, A., Rodríguez-Baños, M., Kobayashi, N., Zayaz, A., Abreu, M., Cantón, R., Del Campo, R., High clonal diversity in a non-outbreak situation of clinical ESBL-producing Klebsiellapneumoniae isolates in the first national surveillance program in Cuba (2014) Microb. Drug Resist., 20, pp. 45-51
  • Wang, G., Huang, T., Surendraiah, P.K.M., Wang, K., Komal, R., Zhuge, J., Chern, C.R., Wormser, G.P., CTX-M p-lactamase-producing Klebsiellapneumoniae in suburban New York, New York, USA (2013) Emerg. Infect. Dis., 19, pp. 1803-1810
  • Johnson, J.R., Johnston, V., Kuskowski, M.A., Nougayrede, J.P., Oswald, E., Molecular epidemiology and phylogenetic distribution of the Escherichia colipks genomic island (2008) J. Clin. Microbiol., 46, pp. 3906-3911